sequencing-by-synthesis technique (i.e. pyrosequencing) Search Results


nanopore sequencing oxford nanopore  (Oxford Nanopore)
90
Oxford Nanopore nanopore sequencing oxford nanopore
Nanopore Sequencing Oxford Nanopore, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing-by-synthesis+technique+(i%2Ee%2E+pyrosequencing)/oxford+nanopore+sequencing/us12205675-820-36-43
Average 90 stars, based on 1 article reviews
nanopore sequencing oxford nanopore - by Bioz Stars, 2026-08
90/100 stars
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minelute reaction cleanup kit  (Qiagen)
96
Qiagen minelute reaction cleanup kit
Minelute Reaction Cleanup Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing-by-synthesis+technique+(i%2Ee%2E+pyrosequencing)/MinElute+Reaction+Cleanup+Kit/pmc03400013-414-28-32
Average 96 stars, based on 1 article reviews
minelute reaction cleanup kit - by Bioz Stars, 2026-08
96/100 stars
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mircute mirna isolation kit  (tiangen biotech co)
96
tiangen biotech co mircute mirna isolation kit
Heatmap showing differentially expressed <t>miRNAs.</t> Heat map shows differentially expressed miRNAs among six libraries with a log 2 Ratio ≥ 1. Bright red and light yellow represent down-regulated and up-regulated miRNAs respectively. a ) 6d-8d, b ) 8d-12d, c ) 12d-15d, d ) 15d-21d, e ) 21d-P6, f ) P48-h6
Mircute Mirna Isolation Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sequencing-by-synthesis+technique+(i%2Ee%2E+pyrosequencing)/miRcute+miRNA+Isolation+Kit/pmc04499447-92-65-69
Average 96 stars, based on 1 article reviews
mircute mirna isolation kit - by Bioz Stars, 2026-08
96/100 stars
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Image Search Results


Heatmap showing differentially expressed miRNAs. Heat map shows differentially expressed miRNAs among six libraries with a log 2 Ratio ≥ 1. Bright red and light yellow represent down-regulated and up-regulated miRNAs respectively. a ) 6d-8d, b ) 8d-12d, c ) 12d-15d, d ) 15d-21d, e ) 21d-P6, f ) P48-h6

Journal: Journal of Ovarian Research

Article Title: Identification of miRNAs during mouse postnatal ovarian development and superovulation

doi: 10.1186/s13048-015-0170-2

Figure Lengend Snippet: Heatmap showing differentially expressed miRNAs. Heat map shows differentially expressed miRNAs among six libraries with a log 2 Ratio ≥ 1. Bright red and light yellow represent down-regulated and up-regulated miRNAs respectively. a ) 6d-8d, b ) 8d-12d, c ) 12d-15d, d ) 15d-21d, e ) 21d-P6, f ) P48-h6

Article Snippet: To validate the differentially expressed miRNAs identified using deep sequencing technology, eight miRNAs were further selected and their relative expression levels were analyzed in different sized follicles (i.e., 100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm isolated from ovary samples of 12d, 21d, P48, and h6, the same as in sequencing samples respectively). miRNA was extracted using miRcute miRNA Isolation Kit (Tiangen, Beijing China) according to manufacturer protocol. cDNA was synthesized using miScript II RT Kit (QIAGEN) and qRT-PCR was performed using the miScript SYBR Green PCR Kit ( QIAGEN ) according to the manufacturer’s protocol.

Techniques:

qRT-PCR validation of eight miRNAs in different sized follicle. qRT–PCR of selected known miRNAs in different size follicles (100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm). miRNA was isolated and cDNA was synthesized from different size follicles followed by qRT-PCR. Capital letters shows expression profile of respective miRNA in deep sequencing data while small letters represents their expression profile in different size follicles using qRT-PCR. A ) Expression profile of mmu-mir-199a in sequencing data. a ) Expression profile of miR-199a through qRT-PCR. B ) Expression profile of mmu-mir-470 in sequencing data. b ) Expression profile of miR-470 through qRT-PCR. C ) Expression profile of mmu-mir-871in sequencing data. c ) Expression profile of miR-871 through qRT-PCR. D ) Expression profile of mmu-mir-351 in sequencing data. d ) Expression profile of miR-351 through qRT-PCR. E ) Expression profile of mmu-mir-191 in sequencing data. e ) Expression profile of miR-191 through qRT-PCR. F ) Expression profile of mmu-mir-34c in sequencing data. f ) Expression profile of miR-34c through qRT-PCR. G ) Expression profile of mmu-let-7a in sequencing data. g ) Expression profile of let-7a through qRT-PCR. H ) Expression profile of mmu-mir-7a in sequencing data. h ) Expression profile of miR-7a through qRT-PCR

Journal: Journal of Ovarian Research

Article Title: Identification of miRNAs during mouse postnatal ovarian development and superovulation

doi: 10.1186/s13048-015-0170-2

Figure Lengend Snippet: qRT-PCR validation of eight miRNAs in different sized follicle. qRT–PCR of selected known miRNAs in different size follicles (100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm). miRNA was isolated and cDNA was synthesized from different size follicles followed by qRT-PCR. Capital letters shows expression profile of respective miRNA in deep sequencing data while small letters represents their expression profile in different size follicles using qRT-PCR. A ) Expression profile of mmu-mir-199a in sequencing data. a ) Expression profile of miR-199a through qRT-PCR. B ) Expression profile of mmu-mir-470 in sequencing data. b ) Expression profile of miR-470 through qRT-PCR. C ) Expression profile of mmu-mir-871in sequencing data. c ) Expression profile of miR-871 through qRT-PCR. D ) Expression profile of mmu-mir-351 in sequencing data. d ) Expression profile of miR-351 through qRT-PCR. E ) Expression profile of mmu-mir-191 in sequencing data. e ) Expression profile of miR-191 through qRT-PCR. F ) Expression profile of mmu-mir-34c in sequencing data. f ) Expression profile of miR-34c through qRT-PCR. G ) Expression profile of mmu-let-7a in sequencing data. g ) Expression profile of let-7a through qRT-PCR. H ) Expression profile of mmu-mir-7a in sequencing data. h ) Expression profile of miR-7a through qRT-PCR

Article Snippet: To validate the differentially expressed miRNAs identified using deep sequencing technology, eight miRNAs were further selected and their relative expression levels were analyzed in different sized follicles (i.e., 100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm isolated from ovary samples of 12d, 21d, P48, and h6, the same as in sequencing samples respectively). miRNA was extracted using miRcute miRNA Isolation Kit (Tiangen, Beijing China) according to manufacturer protocol. cDNA was synthesized using miScript II RT Kit (QIAGEN) and qRT-PCR was performed using the miScript SYBR Green PCR Kit ( QIAGEN ) according to the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Isolation, Synthesized, Expressing, Sequencing

Ovary specific genes targeted by microRNAs. Network shows microRNAs and their predicted target genes. Ovary specific genes are highlighted in the network. Green rectangles represent microRNAs while blue circles represent target genes. The specific interaction is highlighted by red lines between microRNAs and ovary specific target genes

Journal: Journal of Ovarian Research

Article Title: Identification of miRNAs during mouse postnatal ovarian development and superovulation

doi: 10.1186/s13048-015-0170-2

Figure Lengend Snippet: Ovary specific genes targeted by microRNAs. Network shows microRNAs and their predicted target genes. Ovary specific genes are highlighted in the network. Green rectangles represent microRNAs while blue circles represent target genes. The specific interaction is highlighted by red lines between microRNAs and ovary specific target genes

Article Snippet: To validate the differentially expressed miRNAs identified using deep sequencing technology, eight miRNAs were further selected and their relative expression levels were analyzed in different sized follicles (i.e., 100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm isolated from ovary samples of 12d, 21d, P48, and h6, the same as in sequencing samples respectively). miRNA was extracted using miRcute miRNA Isolation Kit (Tiangen, Beijing China) according to manufacturer protocol. cDNA was synthesized using miScript II RT Kit (QIAGEN) and qRT-PCR was performed using the miScript SYBR Green PCR Kit ( QIAGEN ) according to the manufacturer’s protocol.

Techniques:

Pathway annotation. Pathway annotation of differentially expressed miRNAs based on predicted target genes involved in different pathways during 6d-8d ( a ), 8d-12d ( b ), 12d-15d ( c ), 15d-21d ( d ), 21d-P6 ( e ), P48-h6 ( f ). Vertical axis shows pathways while horizontal axis shows number of genes involved in respective pathway

Journal: Journal of Ovarian Research

Article Title: Identification of miRNAs during mouse postnatal ovarian development and superovulation

doi: 10.1186/s13048-015-0170-2

Figure Lengend Snippet: Pathway annotation. Pathway annotation of differentially expressed miRNAs based on predicted target genes involved in different pathways during 6d-8d ( a ), 8d-12d ( b ), 12d-15d ( c ), 15d-21d ( d ), 21d-P6 ( e ), P48-h6 ( f ). Vertical axis shows pathways while horizontal axis shows number of genes involved in respective pathway

Article Snippet: To validate the differentially expressed miRNAs identified using deep sequencing technology, eight miRNAs were further selected and their relative expression levels were analyzed in different sized follicles (i.e., 100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm isolated from ovary samples of 12d, 21d, P48, and h6, the same as in sequencing samples respectively). miRNA was extracted using miRcute miRNA Isolation Kit (Tiangen, Beijing China) according to manufacturer protocol. cDNA was synthesized using miScript II RT Kit (QIAGEN) and qRT-PCR was performed using the miScript SYBR Green PCR Kit ( QIAGEN ) according to the manufacturer’s protocol.

Techniques:

Length distribution and abundance of miRNA sequences in mouse ovary by Illumina deep sequencing. Sequence length distribution of clean reads based on the abundance and distinct sequences; the most abundant size class was 22 nt, followed by 21 nt and 23 nt. a ) 6d, b ) 8d, c ) 12d, d ) 15d, e ) 21d, f ) P6 g ) P48, h ) h6

Journal: Journal of Ovarian Research

Article Title: Identification of miRNAs during mouse postnatal ovarian development and superovulation

doi: 10.1186/s13048-015-0170-2

Figure Lengend Snippet: Length distribution and abundance of miRNA sequences in mouse ovary by Illumina deep sequencing. Sequence length distribution of clean reads based on the abundance and distinct sequences; the most abundant size class was 22 nt, followed by 21 nt and 23 nt. a ) 6d, b ) 8d, c ) 12d, d ) 15d, e ) 21d, f ) P6 g ) P48, h ) h6

Article Snippet: To validate the differentially expressed miRNAs identified using deep sequencing technology, eight miRNAs were further selected and their relative expression levels were analyzed in different sized follicles (i.e., 100 μm −130 μm, 200 μm -280 μm, 450 μm -550 μm, 500 μm -600 μm isolated from ovary samples of 12d, 21d, P48, and h6, the same as in sequencing samples respectively). miRNA was extracted using miRcute miRNA Isolation Kit (Tiangen, Beijing China) according to manufacturer protocol. cDNA was synthesized using miScript II RT Kit (QIAGEN) and qRT-PCR was performed using the miScript SYBR Green PCR Kit ( QIAGEN ) according to the manufacturer’s protocol.

Techniques: Sequencing